2017 年第 12 期 第 12 卷

利用诱导多能干细胞构建类肾体的方法

作者：肖枫林李清刚傅博吴玲玲马倩沈婉君陈香美

英文作者：

单位：100853北京，解放军总医院肾脏病科

英文单位：

关键词：诱导多能干细胞；细胞分化；类肾体

英文关键词：

• 摘要：

• 目的    利用诱导多能干细胞（iPSCs）向肾系细胞定向诱导分化，并获得类肾体。方法    利用肝糖原合成激酶3β受体的选择性抑制剂（CHIR99021）及成纤维细胞生长因子9对iPSCs进行定向诱导分化，控制后肾间充质与输尿管上皮的形成比例，从而构建类肾体。对类肾体细胞进行免疫荧光染色，利用共聚焦激光扫描显微镜观察并拍摄照片。利用扫描电镜观察类肾体的内部结构，并拍摄照片。结果    利用iPSCs分化成功构建出类肾体。通过结构鉴定，证实类肾体中包括肾单位的各个部分。结论    利用iPSCs进行定向诱导分化可以获得包含远端小管、近端小管、肾小球及血管的类肾体。

• Kidney organoids generated from induced pluripotent stem cells

  Xiao Fenglin, Li Qinggang, Fu Bo, Wu Lingling, Ma Qian, Shen Wanjun, Chen Xiangmei

  Department of Nephrology, Chinese PLA General Hospital, Beijing 100853, China

  Corresponding author: Chen Xiangmei, Email: xmchen301@126.com

  【Abstract】Objective    To obtain kidney organoids from induced pluripotent stem cells（iPSCs） differentiation. Methods    Glycogen synthase kinase-3β receptor selective inhibitor CHIR99021 and fibroblast growth factor-9 were used to induce the differentiation of iPSCs and control the ratio between metanephric mesenchyme and ureteral epithelium to construct kidney organoids. Kidney specific markers in kidney organoids were detected through immunofluorescence staining by confocal laser scanning microscope. Internal microstructures of kidney organoids were observed by scanning electron microscope. Results    Kidney organoids containing various parts of nephron were successfully generated from the differentiation of iPSCs. Conclusion    Kidney organoids containing distal and proximal tubules, glomerulus and vasculatures can be obtained by the differentiation of iPSCs.

  【Key words】Induced pluripotent stem cells;Cell differentiation;Kidney organoids

  【Fund program】National Natural Science Foundation of China（81330019）