2018 年第 5 期 第 13 卷

银屑病患者辅助性T细胞17和调节性T细胞及相关细胞因子检测的临床意义

Clinical significances of helper T cell 17, regulatory T cell and the expression of relative cytokines in psoriasis patients

作者：刘正君高美华

英文作者：

单位：266021青岛大学基础医学院免疫学教研室（刘正君现工作单位为解放军第四〇四医院检验科)

英文单位：

关键词：银屑病；辅助性T细胞17；调节性T细胞；白细胞介素

英文关键词：

• 摘要：

• 目的    探讨银屑病患者外周血辅助性T细胞17（Th17）、调节性T细胞（Treg）及相关细胞因子检测的临床意义。方法    选取2016年1—12月解放军第四〇四医院皮肤科收治的银屑病患者60例（银屑病组）及健康体检者30例（对照组），应用流式细胞仪检测外周血中Th17和Treg比例，应用酶联免疫吸附试验方法测定Th17、Treg相关细胞因子白细胞介素(IL)17、IL-10水平。结果    银屑病组Th17比例高于对照组，Treg比例低于对照组，Th17/Treg比值明显高于对照组［（2.03±0.27）%比（0.80±0.32）%、（1.10±0.21）%比（2.80±0.43）%、（1.91±0.46）比（0.30±0.14）］，差异均有统计学意义（均P<0.05）。银屑病组血清IL-17水平明显高于对照组，IL-10水平明显低于对照组［（101±11）ng/L比（56±8）ng/L、（16±3）ng/L比（30±6）ng/L］，差异均有统计学意义（均P<0.05）。结论    银屑病患者中存在Th17及Treg免疫平衡失调。

• 【Abstract】Objective    To investigate the clinical significances of helper T cell 17(Th17), regulatory T cell (Treg) and the expression of relative cytokines in psoriasis patients. Methods    From January to December in 2016, 60 psoriasis patients（psoriasis group） and 30 healthy people（control group） were enrolled in 404 Hospital of the Chinese People′s Liberation Army. Levels of Th17 and Treg were detected by flow cytometry; serum concentrations of interleukin(IL)-17 and IL-10 were detected by enzyme-linked immunosorbent assay. Results    Th17 percentage in psoriasis group was higher than that in control group; Treg percentage in psoriasis group was lower than that in control group; Th17/Treg ratio in psoriasis group was higher than that in control group［（2.03±0.27）% vs （0.80±0.32）%, （1.10±0.21）% vs （2.80±0.43）%, （1.91±0.46） vs （0.30±0.14）］(P<0.05). Serum IL-17 level in psoriasis group was higher than that in control group and serum IL-10 level in psoriasis group was lower than that in control group［（101±11）ng/L vs （56±8）ng/L, （16±3）ng/L vs （30±6）ng/L］(P<0.05). Conclusion    Psoriasis patients may have Th17 and Treg immune balance disorder.

  【Key words】Psoriasis;Helper T cell 17;Regulatory T cell;Interleukin