2018 年第 10 期 第 13 卷

低氧诱导因子1α和轴突导向因子4D与结直肠癌临床病理学特征的相关性研究

Relation among hypoxia inducible factor-1α, semaphorin 4D and clinicopathological characteristics of colorectal carcinoma

作者：郑宏莹蔡克银

英文作者：

单位：430000广州军区武汉总医院干部二科

英文单位：

关键词：结直肠肿瘤；低氧诱导因子1α；轴突导向因子4D

英文关键词：

• 摘要：

• 【摘要】目的    探讨低氧诱导因子1α（HIF-1α）和轴突导向因子4D（Sema4D）在结直肠癌患者癌组织中的表达及其与结直肠癌临床病理学特征的关系。方法    收集2015年7月至2017年7月广州军区武汉总医院手术后结直肠癌组织标本100例（病灶组）和癌旁组织标本50例（癌旁组）。通过免疫组织化学方法检测病灶组和癌旁组标本中HIF-1α、Sema4D蛋白的表达情况并分析其与患者临床病理学特征的关系。结果    病灶组HIF-1α、Sema4D蛋白表达阳性率均明显高于癌旁组［58.0%（58/100）比8.0%（4/50）、60.0%（60/100）比14.0%（7/50）］，差异均有统计学意义（均P＜0.01）。HIF-1α、Sema4D蛋白表达与结直肠癌Dukes分期、淋巴结转移、肿瘤分化程度有密切关系（均P＜0.05）。Spearman相关分析显示，HIF-1a和Sema4D蛋白表达呈正相关（r＝0.642，P＜0.01）。结论    HIF-1α、Sema4D蛋白在结直肠癌组织中高表达，并且与肿瘤的发生发展关系密切，2种蛋白联合检测可为结直肠癌的预后判断提供参考。

• 【Abstract】Objective    To investigate the relation among expressions of hypoxia inducible factor-1α（HIF-1α）, semaphorin 4D（Sema4D） and clinicopathological characteristics of colorectal carcinoma. Methods    From July 2015 to July 2017, 100 specimens of colorectal carcinoma and 50 specimens of paracancerous tissue were collected in Wuhan General Hospital of Guangzhou Military Region. Expressions of HIF-1α and Sema4D were tested by immunohistochemical method. The relation among HIF-1α, Sema4D expression and clinicopathological features of colorectal carcinoma was analyzed. Results    Expression rates of HIF-1α and Sema4D in colorectal carcinoma tissue were significantly higher than those in paracancerous tissue［58.0%（58/100）vs 8.0%（4/50）, 60.0%（60/100）vs 14.0%（7/50）］（P＜0.01）. Expressions of HIF-1α and Sema4D were related to Dukes stage, lymph node metastasis and tumor differentiation(P＜0.05). Spearman analysis showed a positive correlation between HIF-1α and Sema4D expression(r＝0.642, P＜0.01). Conclusions    HIF-1α and Sema4D are highly expressed in colorectal carcinoma and they are closely related to the occurrence and development of tumor. Combined detection of the two proteins may provide a reference for predicting the prognosis of colorectal cancer.