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2019 年第 8 期 第 14 卷

基质细胞衍生因子1和 CXC类趋化因子受体7对β抑制蛋白调控肺癌细胞转移与侵袭作用的影响

Effects of stromal cell-derived factor-1 and CXC chemokine receptor-7 on β-arrestin regulating metastasis and invasion of lung cancer cells

作者:苟志斌朱建勇刘海娟张立波何敏叶青范荣梅李俊敏郭光云

英文作者:

单位:442000湖北省十堰市人民医院湖北医药学院附属人民医院呼吸内科I病区(苟志斌、朱建勇、张立波、何敏、叶青、范荣梅、李俊敏、郭光云),检验科(刘海娟)

英文单位:

关键词:肺癌;基质细胞衍生因子1;CXC类趋化因子受体7;β抑制蛋白

英文关键词:

  • 摘要:
  • 【摘要】目的    探讨基质细胞衍生因子1(SDF-1)和CXC类趋化因子受体7(CXCR7)对β抑制蛋白调控肺癌细胞转移与侵袭作用的影响。方法    取对数生长期肺癌A549细胞系,分别转染CXCR7(CXCR7转染组)、空载质粒(转染对照组),同时设置不进行转染的空白对照组,采用噻唑蓝法检测细胞增殖抑制情况,流式细胞术检测细胞凋亡情况,Transwell实验检测细胞转移与侵袭情况,实时荧光定量聚合酶链反应法检测SDF-1和CXCR7 mRNA的表达,蛋白质印迹法检测蛋白表达情况。结果    转染后48、72 h,CXCR7转染组SDF-1和CXCR7 mRNA相对表达量低于转染对照组和空白对照组(均P<0.05),细胞增殖抑制率、细胞凋亡率均低于转染对照组和空白对照组[转染48 h:(3.2±1.3)%比(10.4±3.3)%、(11.9±3.3)%,(4.4±1.8)%比(16.8±2.2)%、(18.9±3.2)%;转染72 h:(2.1±1.1)%比(14.0±2.1)%、(13.8±1.8)%,(5.4±1.2)%比(18.3±3.7)%、(20.1±2.8)%](均P<0.05)。转染后48 h,CXCR7转染组的细胞转移与侵袭比例以及SDF-1、CXCR7、β抑制蛋白相对表达量均高于转染对照组和空白对照组(均P<0.05)。结论    SDF-1/CXCR7能促进β抑制蛋白的表达,从而促进肺癌细胞增殖、转移与侵袭,抑制细胞凋亡。

  • 【Abstract】Objective    To explore the effects of stromal cell-derived factor-1(SDF-1) and CXC chemokine receptor-7(CXCR7) on metastasis and invasion of lung cancer cells regulated by β-arrestin. Methods    Lung cancer cell line A549 were transfected by CXCR7(CXCR7 group) and empty plasmid(transfection control group); A549 cells with no treatment were blank control group. Cell proliferation was detected by MTT assay; apoptosis was detected by flow cytometry; metastasis and invasion were detected by Transwell assay; SDF-1 and CXCR7 mRNA expression was detected by real-time quantitative polymerase chain reaction; protein expression was detected by western blotting. Results    At 48 and 72 h after transfection, relative expression levels of SDF-1 and CXCR7 mRNA in CXCR7 group were significantly lower than those in transfection control group and blank control group(all P<0.05); cell proliferation inhibition rate and apoptosis rate in CXCR7 group were significantly lower than those in transfection control group and blank control group[48 h: (3.2±1.3)% vs (10.4±3.3)%,(11.9±3.3)%; (4.4±1.8)% vs (16.8±2.2)%,(18.9±3.2)%; 72 h : (2.1±1.1)% vs (14.0±2.1)%,(13.8±1.8)%; (5.4±1.2)% vs (18.3±3.7)%,(20.1±2.8)%](all P<0.05). At 48 h after transfection, cell metastasis rate and invasion rate, relative expression levels of SDF-1, CXCR7 and β-arrestin in CXCR7 group were significantly higher than those in transfection control group and blank control group(all P<0.05). Conclusion    SDF-1/CXCR7 can increase β-arrestin expression and promote proliferation, metastasis and invasion, inhibit apoptosis of lung cancer cells.

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