2020 年第 12 期 第 15 卷

表皮葡萄球菌icaA/icaD基因与前列腺液中性粒细胞弹性蛋白酶检测的临床意义

Clinical significance of detection of Staphylococcus epidermidis icaA/icaD gene and neutrophil elastase in prostatic fluid 

作者：王坤

英文作者：Wang Kun

单位：黄河三门峡医院检验科，河南省三门峡市472000

英文单位：Department of Laboratory Sanmenxia Hospital of the Yellow River Henan Province Sanmenxia 472000 China 

关键词：前列腺炎；表皮葡萄球菌；icaA/icaD基因；中性粒细胞弹性蛋白酶

英文关键词：Prostatitis;Staphylococcusepidermidis;IcaA/icaDgene;Neutrophilelastase

• 摘要：

• 目的 探讨表皮葡萄球菌icaA/icaD基因和前列腺液中性粒细胞弹性蛋白酶（NE）联合检测的临床意义。方法 选取2013年1月至2015年12月在黄河三门峡医院就诊的表皮葡萄球菌性前列腺炎患者657例，收集患者分离出的657株表皮葡萄球菌检验结果以及临床资料。采用聚合酶链反应方法检测表皮葡萄球菌icaA/icaD基因水平，酶联免疫吸附试验法检测前列腺液NE含量。结果 657株表皮葡萄球菌的icaA阳性率为19.6%（129株），icaD阳性率为21.3%（140株）。657例患者中，273例（41.6%）前列腺液中NE含量≤800 μg/L；63例（9.6%）前列腺液中NE含量＞800且＜1 000 μg/L；321例（48.9%）前列腺液中NE含量≥1 000 μg/L。icaA阳性的表皮葡萄球菌感染者中NE≥1 000 μg/L者比例为72.1%（93/129），icaD阳性的表皮葡萄球菌感染者中NE≥1 000 μg/L的比例为72.9%（102/140）。结论 表皮葡萄球菌icaA/icaD基因和前列腺液中NE联合检测对表皮葡萄球菌性前列腺炎诊疗方案的制定有一定的指导意义。

• Objective To investigate the clinical significance of combined detection of Staphylococcus epidermidis icaA/icaD gene and neutrophil elastase (NE) level in prostatic fluid. Methods The clinical data of 657 patients with Staphylococcus epidermidis prostatitis in Sanmenxia Hospital of the Yellow River from January 2013 to December 2015 and detected results of 657 strains Staphylococcus epidermidis from the patients were collected. The icaA/icaD gene level of Staphylococcus epidermidis was detected by polymerase chain reaction, and the content of NE in prostatic fluid was detected by enzyme-linked immunosorbent assay. Results The positive rate of icaA in 657 strains of Staphylococcus epidermidis was 19.6% (129/657), and the positive rate of icaD was 21.3% (140/657). In 273 (41.6%) patients, the content of NE in prostatic fluid was ≤800 μg/L; in 63 patients (9.6%), the content of NE in prostatic fluid was ＞800 and ＜1 000 μg/L; in 321 cases (48.9%), the NE content in prostatic fluid was ≥1 000 μg/L. The proportion of NE≥1 000 μg/L was 72.1%(93/129) in patients with icaA positive Staphylococcus epidermidis infection, and 72.9%(102/140) in patients with icaD positive Staphylococcus epidermidis infection. Conclusion The combined detection of icaA/icaD gene of Staphylococcus epidermidis and NE level in prostatic fluid has certain guiding significance for the development of clinical diagnosis and treatment of prostatitis. style='font-size:16px;font-family:"Calibri","sans-serif"; mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-US;mso-fareast-language: ZH-CN;mso-bidi-language:AR-SA'>0.05). The expression of miR-451a in serum of DLBCL patients was related to Ann Arbor staging and international prognostic index(IPI) score (all P＜0.05). The area under curve of serum miR-451a in the diagnosis of chemotherapy efficacy was 0.816, the sensitivity and specificity were 83.0% and 84.0%, respectively, and the optimal cut-off value was 0.72. Conclusion The expression of miR-451a in serum of DLBCL is down-regulated and related to Ann Arbor staging and IPI score, which has certain application value in the detection of chemotherapy effect.